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4D-BioReconX
4D-BioReconX
  • Installation
  • Basic Usage
  • Tutorials
    • WACCA
      • MIRROR pipeline
      • Detect cell boundaries based on ssDNA images
      • SEAM Pipeline
      • Mesh generation pipeline
    • SPC analysis
      • SPC Pipeline
      • Cell-cell connection estimation
      • CellCellColocation3D Pipeline
      • Meta-Niche
      • Digital Lineage Tracing Pipeline
        • Digital Lineage Tracing
        • Sankey Plot
          • Sankey Plot
    • Polarity analysis
      • Gradient Gene Profiling
      • Spatial pattern Clustering
      • Gradient correlation
      • PCG PCA
    • Other modules
      • Detect blastema by pigment difference
      • Body Straightening
        • Adjust AP-ML Plane
        • Adjust AP-DV Plane
  • Planarian Manuscript
    • Sampling Design
    • Data preprocessing
    • Downstream Analysis
    • Visualization
    • Data availability
    • Index
  • References
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Polarity analysis¶

This section contains main analyses on polarity-related genes to capture positional gradients along the body axis.

  • Gradient Gene Profiling
    • Usage
  • Spatial pattern Clustering
    • Usage
      • Load data in anndata format
      • First round of clustering using hdbscan
      • Second round of “clustering”
      • Save results
      • Visualization by heatmap
  • Gradient correlation
    • Calculating Spearman’s rank correlation coefficient
    • Finding out more polarity-related genes
  • PCG PCA
    • usage
    • example
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Gradient Gene Profiling
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Sankey Plot
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